Here is the full list of all 27 Wacky Spores: The Chase achievements.
Die 25 times
Kill a Boss
Unlock all the mushrooms
Unlock all the artifacts
Unlock all the areas
Run 5000 meters in the Hills
Run 5000 meters in the Desert
Run 5000 meters in the Jungle
Run 5000 meters in the Mountains
Run 5000 meters in the Dungeon
Destroy 50 crates
Pick up 1000 coins
Pick up 25 items
Kill 100 bees
Kill 100 spiders
Freeze 250 enemies
Shrink 250 enemies
Burn 250 enemies
Kill 20 thieves
Kill 20 Treasure Imps
Start 100 runs
Start your first run
Reach level 5
Reach level 50
Reach level 100
Reach level 150
Put 250 enemies to sleep