Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 10 or more buffs during combat.
Play 25 cards in a single turn.
Have 999 Block during combat.
Play 10 Shivs in a single turn.
Defeat a boss in a single turn.
Apply 99 or more Poison on a single enemy.
Defeat a boss without taking any damage.
Exhaust 20 cards in a single combat.
Have 50 or more Strength during combat.
Defeated Donu & Deca.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Beat the game with the Silent.
Defeated the Bronze Automaton.
Defeat 3 enemies with Poison in a single combat.
Defeated the Time Eater.
Defeated the Champ.
Defeated the Guardian.
Win a combat without playing an Attack.
Win a battle with 1 HP remaining.
Have 9 Energy during a single combat.
Beat the game with the Ironclad.
Defeated the Awakened One.
Have 99 or more Block during combat.
Defeated the Collector.
Defeated Hexaghost.
Defeated the Slime Boss.
Obtain all other Achievements.
Finish Donu with a Feed.
Complete Ascension Level 10.
Beat the game with a single relic.
Beat the game in under 20 minutes.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Win a Daily Climb.
Channel 9 Plasma in a single turn.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.