Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Defeat a boss in a single turn.
Play 25 cards in a single turn.
Play 10 Shivs in a single turn.
Have 10 or more buffs during combat.
Have 999 Block during combat.
Have 99 or more Block during combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Have 50 or more Strength during combat.
Apply 99 or more Poison on a single enemy.
Defeat a boss without taking any damage.
Defeated Donu & Deca.
Exhaust 20 cards in a single combat.
Defeat 3 enemies with Poison in a single combat.
Defeated the Awakened One.
Defeated the Bronze Automaton.
Win a combat without playing an Attack.
Beat the game with the Ironclad.
Have 9 Energy during a single combat.
Beat the game with the Silent.
Defeated the Time Eater.
Defeated the Guardian.
Win a battle with 1 HP remaining.
Defeated the Collector.
Defeated the Slime Boss.
Defeated the Champ.
Defeated Hexaghost.
Obtain all other Achievements.
Beat the game in under 20 minutes.
Complete Ascension Level 10.
Beat the game with a single relic.
Finish Donu with a Feed.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.