Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Play 10 Shivs in a single turn.
Exhaust 20 cards in a single combat.
Defeat 3 enemies with Poison in a single combat.
Win a combat without playing an Attack.
Win a battle with 1 HP remaining.
Have 50 or more Strength during combat.
Have 999 Block during combat.
Apply 99 or more Poison on a single enemy.
Have 10 or more buffs during combat.
Have 9 Energy during a single combat.
Defeated the Time Eater.
Defeated the Collector.
Beat the game with the Silent.
Defeated Donu & Deca.
Defeated the Bronze Automaton.
Beat the game with the Ironclad.
Defeated the Awakened One.
Defeat a boss in a single turn.
Play 25 cards in a single turn.
Defeated the Champ.
Defeat a boss without taking any damage.
Defeated the Slime Boss.
Have 99 or more Block during combat.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Beat the game in under 20 minutes.
Finish Donu with a Feed.
Complete Ascension Level 10.
Beat the game with a 5 card deck or smaller.
Beat the game with a single relic.
Unlock Ascension mode.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.