Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Play 10 Shivs in a single turn.
Defeat a boss in a single turn.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Play 25 cards in a single turn.
Have 10 or more buffs during combat.
Have 50 or more Strength during combat.
Defeated the Awakened One.
Have 999 Block during combat.
Beat the game with the Silent.
Defeated Donu & Deca.
Defeat a boss without taking any damage.
Beat the game with the Ironclad.
Defeated the Time Eater.
Apply 99 or more Poison on a single enemy.
Have 9 Energy during a single combat.
Defeated the Bronze Automaton.
Exhaust 20 cards in a single combat.
Win a battle with 1 HP remaining.
Defeated the Collector.
Have 99 or more Block during combat.
Win a combat without playing an Attack.
Defeat 3 enemies with Poison in a single combat.
Defeated the Slime Boss.
Defeated the Champ.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Finish Donu with a Feed.
Beat the game with a 5 card deck or smaller.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Complete Ascension Level 10.
Unlock Ascension mode.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.