Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 999 Block during combat.
Win a combat without playing an Attack.
Have 10 or more buffs during combat.
Play 10 Shivs in a single turn.
Play 25 cards in a single turn.
Defeat a boss without taking any damage.
Have 99 or more Block during combat.
Defeated Donu & Deca.
Apply 99 or more Poison on a single enemy.
Beat the game with the Silent.
Defeated the Champ.
Defeated the Time Eater.
Defeated the Bronze Automaton.
Defeated Hexaghost.
Defeat 3 enemies with Poison in a single combat.
Beat the game with the Ironclad.
Defeated the Awakened One.
Defeat a boss in a single turn.
Defeated the Collector.
Defeated the Slime Boss.
Have 9 Energy during a single combat.
Win a battle with 1 HP remaining.
Defeated the Guardian.
Exhaust 20 cards in a single combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Have 50 or more Strength during combat.
Obtain all other Achievements.
Complete Ascension Level 10.
Unlock Ascension mode.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Beat the game with a 5 card deck or smaller.
Finish Donu with a Feed.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Win a Daily Climb.
Channel 9 Plasma in a single turn.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.