Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Defeat a boss in a single turn.
Play 25 cards in a single turn.
Have 999 Block during combat.
Have 50 or more Strength during combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Have 99 or more Block during combat.
Beat the game with the Ironclad.
Defeated the Time Eater.
Defeated the Collector.
Beat the game with the Silent.
Defeated Donu & Deca.
Have 9 Energy during a single combat.
Play 10 Shivs in a single turn.
Defeated the Champ.
Defeated the Awakened One.
Have 10 or more buffs during combat.
Defeated the Bronze Automaton.
Win a battle with 1 HP remaining.
Exhaust 20 cards in a single combat.
Apply 99 or more Poison on a single enemy.
Defeat a boss without taking any damage.
Defeat 3 enemies with Poison in a single combat.
Defeated the Slime Boss.
Win a combat without playing an Attack.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Complete Ascension Level 10.
Finish Donu with a Feed.
Unlock Ascension mode.
Beat the game with a single relic.
Beat the game in under 20 minutes.
Beat the game with a 5 card deck or smaller.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Win a Daily Climb.
Channel 9 Plasma in a single turn.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.