Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Defeat a boss in a single turn.
Play 25 cards in a single turn.
Have 10 or more buffs during combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Play 10 Shivs in a single turn.
Apply 99 or more Poison on a single enemy.
Have 50 or more Strength during combat.
Defeated the Time Eater.
Have 999 Block during combat.
Defeated the Bronze Automaton.
Win a combat without playing an Attack.
Beat the game with the Silent.
Defeated Donu & Deca.
Have 9 Energy during a single combat.
Defeat 3 enemies with Poison in a single combat.
Defeated the Champ.
Exhaust 20 cards in a single combat.
Win a battle with 1 HP remaining.
Beat the game with the Ironclad.
Defeat a boss without taking any damage.
Defeated the Awakened One.
Have 99 or more Block during combat.
Defeated the Collector.
Defeated the Slime Boss.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Finish Donu with a Feed.
Beat the game with a 5 card deck or smaller.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Complete Ascension Level 10.
Unlock Ascension mode.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.