Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 999 Block during combat.
Defeat a boss in a single turn.
Play 10 Shivs in a single turn.
Defeated the Awakened One.
Play 25 cards in a single turn.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeat a boss without taking any damage.
Beat the game with the Silent.
Defeat 3 enemies with Poison in a single combat.
Apply 99 or more Poison on a single enemy.
Beat the game with the Ironclad.
Defeated the Time Eater.
Have 50 or more Strength during combat.
Defeated Donu & Deca.
Have 9 Energy during a single combat.
Win a combat without playing an Attack.
Have 99 or more Block during combat.
Exhaust 20 cards in a single combat.
Win a battle with 1 HP remaining.
Have 10 or more buffs during combat.
Defeated the Collector.
Defeated the Bronze Automaton.
Defeated the Slime Boss.
Defeated the Champ.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Complete Ascension Level 10.
Finish Donu with a Feed.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Beat the game with a single relic.
Beat the game in under 20 minutes.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.