Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Beat the game with the Ironclad.
Have 10 or more buffs during combat.
Defeated the Time Eater.
Win a combat without playing an Attack.
Play 25 cards in a single turn.
Defeated the Bronze Automaton.
Defeat a boss in a single turn.
Defeated the Awakened One.
Win a battle with 1 HP remaining.
Beat the game with the Silent.
Defeated Donu & Deca.
Have 99 or more Block during combat.
Apply 99 or more Poison on a single enemy.
Defeat 3 enemies with Poison in a single combat.
Play 10 Shivs in a single turn.
Exhaust 20 cards in a single combat.
Defeated the Champ.
Have 9 Energy during a single combat.
Defeat a boss without taking any damage.
Defeated the Collector.
Defeated the Slime Boss.
Defeated Hexaghost.
Defeated the Guardian.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Have 50 or more Strength during combat.
Have 999 Block during combat.
Obtain all other Achievements.
Complete Ascension Level 10.
Unlock Ascension mode.
Beat the game with a single relic.
Beat the game in under 20 minutes.
Beat the game with a 5 card deck or smaller.
Finish Donu with a Feed.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Win a Daily Climb.
Channel 9 Plasma in a single turn.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.