Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 50 or more Strength during combat.
Apply 99 or more Poison on a single enemy.
Defeat 3 enemies with Poison in a single combat.
Beat the game with the Silent.
Have 9 Energy during a single combat.
Defeated the Awakened One.
Win a combat without playing an Attack.
Win a battle with 1 HP remaining.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeated Donu & Deca.
Defeated the Collector.
Exhaust 20 cards in a single combat.
Defeated Hexaghost.
Beat the game with the Ironclad.
Defeated the Time Eater.
Defeated the Champ.
Defeated the Bronze Automaton.
Defeat a boss without taking any damage.
Defeated the Slime Boss.
Defeated the Guardian.
Have 10 or more buffs during combat.
Have 99 or more Block during combat.
Have 999 Block during combat.
Play 10 Shivs in a single turn.
Play 25 cards in a single turn.
Defeat a boss in a single turn.
Obtain all other Achievements.
Unlock Ascension mode.
Beat the game with a single relic.
Beat the game in under 20 minutes.
Beat the game with a 5 card deck or smaller.
Finish Donu with a Feed.
Complete Ascension Level 10.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Win a Daily Climb.
Channel 9 Plasma in a single turn.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.