Here is the full list of all 33 HERO DEFENSE achievements.
Unlock Sam Hain
Reach the Village
Defeat Bones O'Malley
Unlock Barrows
Kill 50,000 Creeps
Kill 500 Creeps with Spells
Fully upgrade the Village
Upgrade every weapon to the highest level
Survive your first encounter with Count Necrosis
Defeat Count Necrosis
Defeat Count Necrosis, once and for all
Defeat the Queen of Corpses
Defeat Dr. Amalgam
Defeat Redfang Wrathborn
Defeat Alana Nightshade
Unlock Jane Doe
Unlock Wylde
Have Jack shoot 10 bolts at once
Kill any boss using only Angry Mobs
Have Barrows slow at least 20 creeps with a single attack
Have all heroes do their Hero's Strike at the same time
Win a map on Insane difficulty with 100% Early Called waves
Have at least one rune of each type
Charge a character to level 10
Complete the "Tower Defense" challenge on Insane difficulty on any map
Complete the "Border Warden" challenge on Insane difficulty on any map
Complete the "Coast Guard" challenge on Insane difficulty on any map
Watch the credits to the end
Hit at least 20 creeps with a single poison spell
Kill a creep that is slowed, poisoned, burning, cursed, scared and cut by razor splinters at the same time
Gather all stars on all maps.
Defeat over 100 waves in the Endless Mode.
Defeat the Ghost Pumpkin.