Here is the full list of all 27 Titan Souls achievements.
Beat the game in under 20 minutes
Trigger the Guardian's switches with the wrong hands and kill it
Beat the game without rolling rolling rolling (in No Roll mode)
Slay all Titans in Iron Mode
Beat the game in Iron Mode
Beat the game in Iron Mode with New Game+
Beat Hard Mode
Knock yourself out in the battle for the Titan Soul
Succumb to the effect of spores for 30 seconds total
Cut all the vines off the Plant Titan
Kill the Knight after destroying all pillars
Produce 50 coins in the Chest fight
Kill the Rolling Titan in under 5 seconds
Drop a bomb on the Lava Blob Titan's head
Remove all teeth from the Mountain Titan then kill it
Destroy all icebergs
Finish in under 10 seconds...
Kill the Broken Statue Titan while pulling back the arrow
Kill the Eye Cube while pulling back the arrow
Kill the Sludge titan with no more possible divisions
Kill the Brain with a flaming arrow
???
Beat the Game
Open the second Sealed Gate
Kill the Skull Titan as it spins
Open the first Sealed Gate
Slay a Titan