Here is the full list of all 24 The Last Clockwinder achievements.
Complete the main story.
Complete the Fuel Generator.
Acquire the infinite Luftapple seed pack.
Meet the highest throughput goal using the required amount of clones in the Medicine 1 room.
Complete the Root Nexus.
Meet the highest throughput goal using the required amount of clones in the Badgertail Farm room.
Acquire the infinite Badgertail seed pack.
Complete the Central Pumping Station.
Acquire the infinite Bomb Berry seed pack.
Meet the highest throughput goal using the required amount of clones in the Bomb Berry Laboratory room.
Acquire the infinite Blusher seed pack.
Create the maximum allowed number of Gardeners in a single room.
Meet the highest throughput goal using the required amount of clones in the Blusher Garden room.
Meet the highest throughput goal using the required amount of clones in the Bomb Berries room.
Meet the highest throughput goal using the required amount of clones in the Blusher Laboratory room.
Meet the highest throughput goal using the required amount of clones in the Lilypads room.
Meet the highest throughput goal using the required amount of clones in the Dewshots room.
Meet the highest throughput goal using the required amount of clones in the Badgertail Laboratory Room.
Meet the highest throughput goal using the required amount of clones in the Luftapples room.
Meet the highest throughput goal using the required amount of clones in the Luftapple Laboratory room.
Meet the highest throughput goal using the required amount of clones in the Medicine 3 room.
Meet the highest throughput goal using the required amount of clones in the Medicine 2 room.
Drop a vital object off the side of the Clocktower.
Play a certain melody on the piano.