Here is the full list of all 46 The Amazing Spider-Man achievements.
Destroy a Seeker before it can call a Hunter
Clear the first fight against the Hunter robots
Defeat a Hunter without using your Web-Shooters
Defeat Vermin
Defeat 100 enemies
Perform 25 Signature Moves
Defeat Rhino in the city
Defeat the S-01
Complete the game on human difficulty
Complete the game on hero difficulty
Complete the game on super hero difficulty
Collect all 700 Spider-Man Comic Pages
Collect all Magazines
Collect all hidden Tech Pieces
Collect all Oscorp Manuals
Collect all Audio Evidence
Achieve a combo streak of 42
Perform a Web-Rush punch
Immobilize 6 enemies simultaneously with web
Defeat 50 enemies by performing Stealth Takedowns
Defeat 100 enemies by performing Stealth Takedowns
Clear the second fight against the Hunter robots
Defeat the S-02
Defeat the S-03
Defeat the S-01 without touching the ground
Rescue the civilian in the train docking station
Rescue Alistaire Smythe
Win the showdown in the water treatment facility
Defeat Scorpion in Oscorp tower
Capture the cat burglar
Defeat Nattie
Defeat Lizard
Defeat 500 enemies
Defeat 1000 enemies
Acquire all upgrades
Unlock all concept art
Chain 10 Web-Rushes in the city
Rescue all infected civilians
Return all escapees to the police
Clear all car chases
Resolve all police deadlocks
Save all hostages caught in petty crimes
Complete all XTreme Video challenges
Complete all XTreme Race challenges
Complete all Oscorp Secret Research Labs
Defeat Scorpion in the city