Here is the full list of all 48 Subterrain achievements.
Survive for 24 hours in a single game.
Discover the 3D printing facilities of Nilson Engineering.
Complete the tutorial section of the game at MPO Prison.
Kill 10 enemies of any type.
Survive for more than 72 hours in a single game.
Kill 50 enemies of any type.
Kill 100 enemies of any type.
Collect ore 50 times using the extractor at MPO's mine.
Explore every floor and every district of MPO.
Use toilets 30 times in one game.
Destroy 50 cocoons before enemies pop out of them.
Kill 30 Big Fat enemies.
Survive for more than 120 hours in a single game.
Kill 40 Spitter enemies.
Kill 80 Brain Bug enemies.
Collect ore 10 times using the extractor at MPO's mine.
Collect ore 100 times using the extractor at MPO's mine.
Complete 10 research projects at Herbert Research.
Complete 20 research projects at Herbert Research.
Complete 50 research projects at Herbert Research.
Produce 10 items using 3D printer or bio combiner in Nilson Engineering.
Produce 30 items using the 3D printer or bio combiner at Nilson Engineering.
Produce 80 items using 3D printer or bio combiner in Nilson Engineering.
Produce fruits or veggies 10 times at MPO's biosphere.
Produce fruits or veggies 50 times at MPO's biosphere.
Produce fruits or veggies 100 times at MPO's biosphere.
Discover the Aries residential district.
Discover the agricultural facilities of MPO's biosphere.
Discover the sprawling business of Mr. Bigelow: the Solar Casino.
Discover the Gemini residential district.
Discover Dr. West's old workplace: the Herbert Research Center.
Discover the business of the Burlington family: the Carson Hotel.
Discover the Leo residential district.
Discover the ore extraction facilities of MPO's mine.
Discover the Pisces residential district.
Discover the shopping district of MPO: Spirit Plaza.
Discover the space port for MPO: the Murray Space Center.
Discover a residential district of MPO: Viking Square.
Beat the game using the "Easy Mode" or higher difficulty setting.
Beat the game using the "Normal Mode" or higher difficulty setting.
Beat the game using the "Hardcore Mode" or higher difficulty setting.
Beat the game using the "Nightmare Mode" difficulty setting.
Beat the game with the "Permanent Death" option enabled.