Here is the full list of all 47 Resident Evil 0 achievements. It takes around 40-50 hours to unlock all of the achievements on Windows.
Finish the game without using a First-Aid Spray.
Defeat Queen Leech.
Complete the treatment plant.
Obtain all keys and use them all.
Defeat the Proto Tyrant.
Complete the factory.
Fight off the Proto Tyrant.
Complete the laboratory.
Use a Green Chemical.
Defeat Infected Bat.
Complete the training facility.
Defeat a zombie by stomping on them.
Try to push something heavy with Rebecca.
Defeat Centurion.
Create a Molotov Cocktail.
Complete the train.
Defeat an enemy by burning them.
Defeat an enemy with a critical-hit headshot.
Change into a costume.
Defeat Stinger.
Finish the game with rank S.
Finish the game on Hard difficulty.
Finish the game without combining herbs.
Finish the game without saving.
Finish the game without using a recovery item (including Green Chemicals).
Defeat all bosses except Centurion using Rebecca.
Attack an enemy to save your partner who is grappling with that enemy.
Defeat an enemy with a knife.
Destroy a zombie's legs.
Shoot down a Hunter or an Eliminator while they are jump attacking in the air.
Break free of a Cerberus while they are biting you or trying to push you down.
Create a Custom Handgun.
Defeat Mimicry Marcus with a Molotov Cocktail or a Napalm Grenade.
Read all files.
Defeat Mimicry Marcus for the first time only using a knife.
Combine every type of herb with each other.
Obtain all weapons.
Defeat 16 bats in the church.
Put on Team Wesker costume.
Play the piano using Rebecca.
Get picked up by the Infected Bat.
Play Leech Hunter.
Finish Leech Hunter with rank A.
Finish Leech Hunter with rank E or above.
Collect all of only one color of Leech Charms.
Defeat all enemies in Leech Hunter.
Kill 50 enemies with the Death Stare at full charge.