Here is the full list of all 47 Resident Evil 0 achievements. It takes around 40-50 hours to unlock all of the achievements on Windows.
Finish the game on Hard difficulty.
Read all files.
Combine every type of herb with each other.
Finish the game without combining herbs.
Finish the game without using a First-Aid Spray.
Finish the game without using a recovery item (including Green Chemicals).
Destroy a zombie's legs.
Defeat Mimicry Marcus for the first time only using a knife.
Obtain all weapons.
Finish the game with rank S.
Finish the game without saving.
Defeat all bosses except Centurion using Rebecca.
Put on Team Wesker costume.
Defeat 16 bats in the church.
Kill 50 enemies with the Death Stare at full charge.
Defeat Mimicry Marcus with a Molotov Cocktail or a Napalm Grenade.
Defeat all enemies in Leech Hunter.
Collect all of only one color of Leech Charms.
Finish Leech Hunter with rank A.
Play Leech Hunter.
Finish Leech Hunter with rank E or above.
Shoot down a Hunter or an Eliminator while they are jump attacking in the air.
Defeat Queen Leech.
Complete the treatment plant.
Obtain all keys and use them all.
Defeat the Proto Tyrant.
Complete the factory.
Fight off the Proto Tyrant.
Complete the laboratory.
Defeat Infected Bat.
Defeat an enemy by burning them.
Get picked up by the Infected Bat.
Complete the training facility.
Play the piano using Rebecca.
Create a Custom Handgun.
Defeat a zombie by stomping on them.
Use a Green Chemical.
Try to push something heavy with Rebecca.
Defeat Centurion.
Attack an enemy to save your partner who is grappling with that enemy.
Complete the train.
Change into a costume.
Create a Molotov Cocktail.
Defeat an enemy with a critical-hit headshot.
Defeat an enemy with a knife.
Defeat Stinger.
Break free of a Cerberus while they are biting you or trying to push you down.