Full list of all 54 Enter the Gungeon achievements. It takes around 120-150 hours to unlock all of the achievements in the base game on Windows.
The base game contains 48 achievements, and there are 2 DLC packs containing 6 achievements.
Complete the game with the Sorceress's Enchanted Gun
Kill 100 enemies by dropping chandeliers
Kill 100 enemies while riding in a mine cart
Complete Frifle's Challenges
Collect five Master Rounds in one run
Open the shortcut to the Fifth Chamber
Open the shortcut to the Fourth Chamber
Carry a large amount of money at once
Ace Winchester's game 3 times
Complete the game with Beast Mode on
Unlock the Bullet
Clear the Sixth Chamber
Clear the Fifth Chamber 10 Times
Steal 10 things
Complete all 4 main character Pasts
Kill the Hunter's Past
Fall at the last second
Kill the Convict's Past
Unlock the Robot
Kill the Pilot's Past
Kill the Marine's Past
Clear the Fourth Chamber 20 Times
Die in the Past
Construct the Bullet that can kill the Past
Map the first Five Chambers for the lost adventurer
Populate the Breach
You've met with a terrible fate, haven't you
Kill a Jammed Boss
Deliver the Golem's replacement arm
Kill a frozen enemy by rolling into it
Avenge Manuel
Defeat the Boss of the Fifth Chamber
Accessed the Hidden Market
Clear the Third Chamber 30 Times
Open the shortcut to the Third Chamber
Access the Temple
Win a wager against the Gunsling King 5 times
Clear the Second Chamber 40 Times
Always be flipping. Guns are for flippers
Kill a boss after covering it with glitter
Clear the First Chamber 50 Times
Kill 100 enemies by knocking them into pits
Spend big at the Acquisitions Department
Get the jump on a Mimic
Access the Oubliette
Why
Open the shortcut to the Second Chamber
Complete the Tutorial
Complete Daisuke's trial
Kill the Robot's Past
Kill the Bullet's Past
Defeat an Advanced Boss
Take Revenge
Complete Tonic's Challenge