Full list of all 54 Enter the Gungeon achievements. It takes around 120-150 hours to unlock all of the achievements in the base game on Windows.
The base game contains 48 achievements, and there are 2 DLC packs containing 6 achievements.
Unlock the Robot
Unlock the Bullet
Kill a Jammed Boss
Clear the Sixth Chamber
Map the first Five Chambers for the lost adventurer
Steal 10 things
Always be flipping. Guns are for flippers
Clear the Fifth Chamber 10 Times
Open the shortcut to the Third Chamber
Carry a large amount of money at once
Complete all 4 main character Pasts
Kill the Pilot's Past
Kill the Convict's Past
Complete the game with Beast Mode on
Ace Winchester's game 3 times
Accessed the Hidden Market
Deliver the Golem's replacement arm
Kill the Marine's Past
Clear the Fourth Chamber 20 Times
Kill the Hunter's Past
Construct the Bullet that can kill the Past
Clear the Third Chamber 30 Times
Access the Temple
Open the shortcut to the Second Chamber
Kill a frozen enemy by rolling into it
Kill a boss after covering it with glitter
Fall at the last second
Clear the Second Chamber 40 Times
Clear the First Chamber 50 Times
Kill 100 enemies by knocking them into pits
Populate the Breach
Avenge Manuel
Why
Defeat the Boss of the Fifth Chamber
Win a wager against the Gunsling King 5 times
Get the jump on a Mimic
Spend big at the Acquisitions Department
Access the Oubliette
Complete the Tutorial
Collect five Master Rounds in one run
Complete the game with the Sorceress's Enchanted Gun
Complete Frifle's Challenges
Open the shortcut to the Fourth Chamber
Open the shortcut to the Fifth Chamber
Kill 100 enemies by dropping chandeliers
Kill 100 enemies while riding in a mine cart
Die in the Past
You've met with a terrible fate, haven't you
Kill the Robot's Past
Kill the Bullet's Past
Complete Daisuke's trial
Defeat an Advanced Boss
Take Revenge
Complete Tonic's Challenge