Full list of all 54 Enter the Gungeon achievements. It takes around 120-150 hours to unlock all of the achievements in the base game on Windows.
The base game contains 48 achievements, and there are 2 DLC packs containing 6 achievements.
Complete Frifle's Challenges
Populate the Breach
Unlock the Bullet
Kill 100 enemies by dropping chandeliers
Ace Winchester's game 3 times
Open the shortcut to the Fifth Chamber
Always be flipping. Guns are for flippers
Collect five Master Rounds in one run
Avenge Manuel
Open the shortcut to the Fourth Chamber
Complete the game with Beast Mode on
Kill 100 enemies while riding in a mine cart
Unlock the Robot
Why
Clear the Sixth Chamber
Clear the Fifth Chamber 10 Times
Complete all 4 main character Pasts
Kill the Hunter's Past
Kill a frozen enemy by rolling into it
Kill the Marine's Past
Clear the Fourth Chamber 20 Times
Open the shortcut to the Third Chamber
Die in the Past
You've met with a terrible fate, haven't you
Kill a Jammed Boss
Clear the Third Chamber 30 Times
Kill the Pilot's Past
Complete the game with the Sorceress's Enchanted Gun
Access the Temple
Kill the Convict's Past
Construct the Bullet that can kill the Past
Map the first Five Chambers for the lost adventurer
Steal 10 things
Deliver the Golem's replacement arm
Carry a large amount of money at once
Clear the Second Chamber 40 Times
Win a wager against the Gunsling King 5 times
Accessed the Hidden Market
Kill 100 enemies by knocking them into pits
Clear the First Chamber 50 Times
Open the shortcut to the Second Chamber
Fall at the last second
Defeat the Boss of the Fifth Chamber
Spend big at the Acquisitions Department
Kill a boss after covering it with glitter
Access the Oubliette
Complete the Tutorial
Get the jump on a Mimic
Complete Daisuke's trial
Kill the Bullet's Past
Kill the Robot's Past
Defeat an Advanced Boss
Complete Tonic's Challenge
Take Revenge