Here is the full list of all 50 Eldest Souls achievements.
Have at least 10 Windleaves active at once.
Defeat Eksyll in Abyss Mode.
Gain the Kindled Heart of Fire
Defeat Aryana in Abyss Mode.
Maintain 4 stacks of Berserker for 30s.
Defeat The Warden of Purity in Abyss Mode.
Defeat The Deer God in Abyss Mode.
Defeat Drakmur in Abyss Mode.
Defeat Hyem in Abyss Mode.
Defeat Eos in Abyss Mode.
Defeat Azikel in Abyss Mode.
Defeat the Guardian in Abyss Mode.
Die to a rolling log.
Defeat the Watchdog while taking 0 damage.
Defeat Eksyll
Reach the Steps of Ascension
Gain the Blessed Feather of Dawn
Gain the Abyssal Eye
Obtain the Empty Vessel
Defeat Aryana, First of the Moon.
Defeat The Deer God and the Warden of Purity.
Deliver the bag of crowns to Edelon.
Defeat Drakmur, the Nightmare
Hit enemies with a Shard attack 200 times
Hit enemies with a thrust attack 200 times
Defeat Hyem of the Frigid Waste.
Perform 3 Perfect Counters in a row against any Boss.
Reach Concord Sanctuary
Defeat Azikel, God of Light.
Defeat Eos, God of Unity
Retry a Boss 10 times in a row.
Hit an enemy with Windslide, Berserker Slash, or Counter 20 times.
Reach the Crossroads
Defeat the Guardian.
Die to a Boss.
Talk with Rass
Deliver the harp string to Edd
Defeat the Watchdog.
Reach Crusader's Landing
Defeat the Guardian while taking 0 damage.
Defeat Azikel, God of Light while taking 0 damage.
Defeat Eos, God of Unity while taking 0 damage.
Defeat Hyem while taking 0 damage.
Defeat The Deer God and the Warden of Purity while taking 0 damage.
Defeat Aryana, First of the Moon while taking 0 damage.
Defeat Drakmur, the Nightmare while taking 0 damage
Reach the ending without dying once.
Finish the game without activating any Shard or Infusions.
Gain the Blessed Trueice Prism
Gain the Blessed Hunter's Charm