Here is the full list of all 50 Eldest Souls achievements.
Defeat Eos, God of Unity while taking 0 damage.
Defeat Azikel, God of Light while taking 0 damage.
Reach the ending without dying once.
Defeat Hyem while taking 0 damage.
Finish the game without activating any Shard or Infusions.
Gain the Blessed Hunter's Charm
Perform 3 Perfect Counters in a row against any Boss.
Have at least 10 Windleaves active at once.
Defeat Eksyll in Abyss Mode.
Defeat the Watchdog while taking 0 damage.
Gain the Abyssal Eye
Defeat Drakmur, the Nightmare while taking 0 damage
Defeat the Guardian while taking 0 damage.
Defeat The Deer God and the Warden of Purity while taking 0 damage.
Defeat Aryana, First of the Moon while taking 0 damage.
Gain the Kindled Heart of Fire
Defeat Aryana in Abyss Mode.
Defeat The Warden of Purity in Abyss Mode.
Defeat The Deer God in Abyss Mode.
Defeat Drakmur in Abyss Mode.
Defeat Hyem in Abyss Mode.
Maintain 4 stacks of Berserker for 30s.
Defeat Eos in Abyss Mode.
Defeat Azikel in Abyss Mode.
Defeat the Guardian in Abyss Mode.
Die to a rolling log.
Defeat Eksyll
Reach the Steps of Ascension
Gain the Blessed Trueice Prism
Deliver the bag of crowns to Edelon.
Gain the Blessed Feather of Dawn
Obtain the Empty Vessel
Defeat Drakmur, the Nightmare
Defeat Hyem of the Frigid Waste.
Defeat Aryana, First of the Moon.
Defeat The Deer God and the Warden of Purity.
Hit enemies with a Shard attack 200 times
Hit enemies with a thrust attack 200 times
Reach Concord Sanctuary
Defeat Eos, God of Unity
Defeat Azikel, God of Light.
Retry a Boss 10 times in a row.
Hit an enemy with Windslide, Berserker Slash, or Counter 20 times.
Reach the Crossroads
Defeat the Guardian.
Die to a Boss.
Talk with Rass
Deliver the harp string to Edd
Defeat the Watchdog.
Reach Crusader's Landing