Here is the full list of all 50 Eldest Souls achievements.
Reach the ending without dying once.
Gain the Abyssal Eye
Defeat Drakmur, the Nightmare while taking 0 damage
Defeat Azikel, God of Light while taking 0 damage.
Defeat Eksyll in Abyss Mode.
Deliver the bag of crowns to Edelon.
Gain the Blessed Hunter's Charm
Defeat Drakmur in Abyss Mode.
Perform 3 Perfect Counters in a row against any Boss.
Defeat Aryana in Abyss Mode.
Have at least 10 Windleaves active at once.
Defeat The Warden of Purity in Abyss Mode.
Defeat The Deer God in Abyss Mode.
Maintain 4 stacks of Berserker for 30s.
Defeat Hyem in Abyss Mode.
Defeat Azikel in Abyss Mode.
Defeat Eos in Abyss Mode.
Defeat the Guardian in Abyss Mode.
Defeat the Watchdog while taking 0 damage.
Defeat Eksyll
Reach the Steps of Ascension
Gain the Blessed Trueice Prism
Obtain the Empty Vessel
Defeat The Deer God and the Warden of Purity.
Defeat Drakmur, the Nightmare
Defeat Hyem of the Frigid Waste.
Gain the Blessed Feather of Dawn
Defeat Aryana, First of the Moon.
Reach Concord Sanctuary
Defeat Eos, God of Unity
Defeat Azikel, God of Light.
Reach the Crossroads
Defeat the Guardian.
Hit an enemy with Windslide, Berserker Slash, or Counter 20 times.
Hit enemies with a thrust attack 200 times
Retry a Boss 10 times in a row.
Talk with Rass
Deliver the harp string to Edd
Defeat the Watchdog.
Die to a Boss.
Die to a rolling log.
Reach Crusader's Landing
Defeat the Guardian while taking 0 damage.
Defeat Eos, God of Unity while taking 0 damage.
Defeat Hyem while taking 0 damage.
Defeat The Deer God and the Warden of Purity while taking 0 damage.
Defeat Aryana, First of the Moon while taking 0 damage.
Finish the game without activating any Shard or Infusions.
Hit enemies with a Shard attack 200 times
Gain the Kindled Heart of Fire