Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 999 Block during combat.
Defeat a boss in a single turn.
Apply 99 or more Poison on a single enemy.
Win a combat without playing an Attack.
Defeat 3 enemies with Poison in a single combat.
Have 99 or more Block during combat.
Have 10 or more buffs during combat.
Play 25 cards in a single turn.
Play 10 Shivs in a single turn.
Defeated the Awakened One.
Exhaust 20 cards in a single combat.
Have 9 Energy during a single combat.
Defeat a boss without taking any damage.
Beat the game with the Silent.
Win a battle with 1 HP remaining.
Defeated Donu & Deca.
Beat the game with the Ironclad.
Defeated the Time Eater.
Have 50 or more Strength during combat.
Defeated the Collector.
Defeated the Slime Boss.
Defeated the Bronze Automaton.
Defeated Hexaghost.
Defeated the Champ.
Defeated the Guardian.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Obtain all other Achievements.
Finish Donu with a Feed.
Beat the game with a single relic.
Beat the game with a 5 card deck or smaller.
Beat the game in under 20 minutes.
Complete Ascension Level 10.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.