Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Play 10 Shivs in a single turn.
Have 999 Block during combat.
Win a combat without playing an Attack.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeat a boss in a single turn.
Have 50 or more Strength during combat.
Win a battle with 1 HP remaining.
Apply 99 or more Poison on a single enemy.
Exhaust 20 cards in a single combat.
Have 9 Energy during a single combat.
Defeated the Time Eater.
Defeat a boss without taking any damage.
Have 99 or more Block during combat.
Defeat 3 enemies with Poison in a single combat.
Defeated the Champ.
Beat the game with the Silent.
Defeated Donu & Deca.
Have 10 or more buffs during combat.
Beat the game with the Ironclad.
Defeated the Awakened One.
Defeated the Collector.
Defeated the Slime Boss.
Defeated the Bronze Automaton.
Defeated Hexaghost.
Defeated the Guardian.
Play 25 cards in a single turn.
Obtain all other Achievements.
Complete Ascension Level 10.
Beat the game with a 5 card deck or smaller.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Unlock Ascension mode.
Finish Donu with a Feed.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Win a Daily Climb.
Channel 9 Plasma in a single turn.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.